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Bacteriology

Specimen Type Test Requisition Container            Notes                                           
1st Morning Urine Mycobacteria (AFB) MS 331

Complete first morning specimen in a sterile container.

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week; Final: 4 - 8 weeks
Notes: N/A

Aspirate Mycobacteria (AFB) MS 331

 Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 4 - 8 weeks
Notes:N/A

Bartholin's gland abscess Culture and sensitivity (C&S) MS 349

 Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A

Bile of duodenal aspirate Culture and sensitivity (C&S) MS 349

Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes:N/A

 Blood from adult Culture and sensitivity (C&S)  MS 349

9 mL of blood in BacT/Alert aerobic bottle and BacT/Alert anaerobic bottle

Specimen Holding Temperature: 35°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results:  5 days unless positive
Notes: 2 sets of blood cultures are recommended for routine culture but no more than 3 sets are generally required.  Each set of blood cultures should be drawn from a separate site and inoculated into one aerobic and one anaerobic bottle. Positive blood culture

Blood from adult Fungus culture (Histoplasma/Blastomyces)  MS 331

9 mL of blood in 10 mL Yellow/Black top Isolator 10 tube

Specimen Holding Temperature: Room Temperature

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 4 weeks unless positive
Notes: Positive blood culture results are phoned immediately. Isolator tubes are available from Microbiology department, room 1470.

Blood from adult  Mycobacteria (AFB)  MS 331

9 mL of blood in 10 mL Green top, sodium heparin

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week; Final: 4 - 8 weeks
Notes: Positive blood culture results are phoned immediately.
Blood from adult Sub-acute bacterial endocarditis (SBE) MS 349

9 mL of blood in BacT/Alert FAN aerobic bottle and BacT/Alert FAN anaerobic bottle

Specimen Holding Temperature: 35°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 3 weeks unless positive
Notes: Positive blood culture results are phoned immediately.

Blood from neonate Culture and sensitivity (C&S) MS 349

BacT/Alert, up to 1 mL in pediatric bottle

Specimen Holding Temperature: 35°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Prelim: 48 hours; Final: 5 days; unless positive
Notes: Positive blood culture results are phoned immediately.

Bronchial brushings Culture and sensitivity (C&S) MS 349

Bijou bottle with 1 mL Ringer's lactate

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Prelim: 24 hours;  Final: 48 hours
Notes: N/A

 Bronchoalveolar lavage (BAL) Chlamydia and Mycoplasma pneumonia  MS 331

Sterile container, 1 mL

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 4 weeks
Notes: N/A
Bronchoalveolar lavage (BAL) Culture and sensitivity (C&S) MS 349

Sterile container, 15 mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A

Bronchoalveolar lavage (BAL) Fungus culture MS 331

Sterile container, 1 mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes: N/A
Bronchoalveolar lavage (BAL) Legionella culture MS 331

 Sterile container, 1 mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 2 - 3 weeks
Notes: N/A
Bronchoalveolar lavage (BAL) Mycobacteria (AFB) MS 331

 Sterile container, 5 mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 4 - 8 weeks
Notes: N/A
Bronchoscopy aspirates, washings or lung needle aspirates Chlamydia and Mycoplasma pneumonia MS 331

Sterile container, 1mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 4 weeks
Notes: N/A
Bronchoscopy aspirates, washings or lung needle aspirates Culture and sensitivity (C&S) MS 349

Sterile container, 15 mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
Bronchoscopy aspirates, washings or lung needle aspirates Fungus culture MS 331

Sterile container, 1mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes: N/A
Bronchoscopy aspirates, washings or lung needle aspirates Legionella culture MS 331

Sterile container, 1mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 2 - 3 weeks
Notes: N/A
Bronchoscopy aspirates, washings or lung needle aspirates Mycobacteria (AFB) MS 331

Sterile container, 5mL

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 4 - 8 weeks
Notes: N/A
Cervical or urethral swab Culture and sensitivity (C&S) MS 349

Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 24 - 48 hours
Notes: Specimens examined for N. gonorrhoeae only. Tests for Mycoplasma or Ureaplasma not available.
Conjuctival swab and lids Culture and sensitivity (C&S) MS 349

Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes:N/A

Conjuctival swab and lids Viral Detection MS 331

Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 24 hours - 1 week
Notes: N/A

Corneal scraping Culture and sensitivity (C&S) MS 349

 1 - 3 smear(s); inoculate culture media

Specimen Holding Temperature: 4°C for media, Room Temperature for slides

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 5 days
Notes: N/A
Corneal scraping Fungus culture MS 331

 1 - 3 smear(s); inoculate culture media

Specimen Holding Temperature: 4°C for media, Room Temperature for slides

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours; Final: 4 weeks
Notes:N/A
Deep wound and aspirate Anaerobic culture MS 331

 Swab in asprirate in anaerobic transport containers

Specimen Holding Temperature: Room Temperature

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 48 - 72 hours
Notes: N/A

Deep wound and aspirate Culture and sensitivity (C&S) MS 349

 Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
Deep wound and aspirate Fungus culture MS 331

Copan Eswab 

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes: N/A
Ear swab Culture and sensitivity (C&S) MS 349

 Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A

Ear swab Fungus culture MS 331

 Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours; Final: 4 weeks
Notes: N/A

Epiglottal swab Culture and sensitivity (C&S) MS 349

 Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
Faeces (Stool) Culture and sensitivity (C&S) MS 349

 Enteric Pathogen Transport (EPT) Media

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 48 - 72 hours
Notes:N/A
Faeces (Stool) VRE MS 349

 Enteric pathogen transport (EPT) media or sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 48 - 72 hours
Notes:N/A
 Genital ulcer specimen  Culture for H. ducreyi (chancroid)  MS 349

Copan Eswab

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 1 week
Notes: Contact medical microbiologist if chancroid or syphilis is suspected.
Heart value (tissue/biopsy) Mycoplasma MS 331

Sterile container with sterile saline

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 4 weeks
Notes: N/A
Heart value (tissue/biopsy) Mycoplasma (AFB) MS 331

Sterile container with sterile saline

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 4 weeks
Notes: N/A
Heart value (tissue/biopsy) Culture and sensitivity (C&S) MS 349

Sterile container with sterile saline

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
Heart value (tissue/biopsy) Fungus culture MS 331

Sterile container with sterile saline

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes: N/A
Heart valve (tissue/biopsy) Legionella culture MS 331

Sterile container with sterile saline

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 4 days;  Final: 2 - 3 weeks
Notes: N/A
Heart valve (tissue/biopsy) Mycobacteria (AFB) MS 331

Sterile container with sterile saline

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 4 - 8 weeks
Notes: N/A
Intraocular aspirate (anterior / aqueous chamber vitreous fluid) Culture and sensitivity (C&S) MS 349

 Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes:N/A
Intraocular aspirate (anterior / aqueous chamber vitreous fluid) Fungus culture MS 331

 Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours; Final: 4 weeks
Notes:N/A
Intraocular aspirate (anterior / aqueous chamber vitreous fluid) Mycobacteria (AFB) MS 331

 Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 4 - 8 weeks
Notes:N/A
Intra-uterine device Examination for Actinomyces MS 349

Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 1 hour
Notes: IUD's are not cultured. A gram smear examination only is performed for the detection of Actinomyces.
Intravascular catheter tip Culture and sensitivity (C&S) MS 349

Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 48 - 72 hours
Notes: N/A
Lacrimal stone (tear duct) Culture and sensitivity (C&S) MS 349

Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes:N/A
 Lacrimal stone (tear duct)  Fungus culture  MS 331

Sterile container

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 4 weeks
Notes:N/A

 Lacrimal stone (tear duct)  Mycobacteria (AFB)  MS 331

Sterile container

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week; Final: 4 - 8 weeks
Notes:N/A
Mid-stream urine (MSU) Culture and sensitivity (C&S) MS 349

 Grey top BD urine vacutainer tube.

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 24 - 48 hours
Notes: N/A
Mid-stream urine (MSU) Eosinophil count MS 331

Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Same day
Notes: N/A
Mid-stream urine (MSU) Fungus culture MS 331

Grey top BD urine vacutainer tube.

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes: Urine specimens requesting C&S will be examined for Candida. If other fungi are suspected, they must be specified.
Mouth swab Fungus culture MS 349

 Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Same day
Notes: N/A
Nasopharyngeal or nasal swab B. pertussis MS 331

 Bordetella pertussis Kit. PCR transport medium is colourless. Do not use viral transport medium which is pink in color.

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 2 weeks
Notes: Maximum storage / transit time at 20C to 80C is within 5 days of collection.
Prostatic fluid, seminal fluid or sperm Culture and sensitivity (C&S) MS 349

Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
 Rectal swab  Culture and sensitivity (C&S), VRE, MRSA  MS 349

Cobas Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
Sinus or antral lavage Culture and sensitivity (C&S) MS 349

 Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes:N/A
Sinus or antral lavage Fungus culture MS 331

 Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes:N/A
Spinal fluid (CSF) or other sterile body fluids Cryptococcal antigen MS 331

Sterile container

Specimen Holding Temperature: Room temperature - 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 24 hours
Notes: Test suitable for CSF and serum only

Spinal fluid (CSF) or other sterile body fluids Culture and sensitivity (C&S) MS 349

Sterile container

Specimen Holding Temperature: Room temperature

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 24 - 48 hours
Notes: If multiple tube of CSF are collected, the first tube shall  not  be used for microbiological examination.
Spinal fluid (CSF) or other sterile body fluids Fungus culture MS 331

Sterile container

Specimen Holding Temperature: Room temperature - 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes: N/A
Spinal fluid (CSF) or other sterile body fluids Mycobacteria (AFB) MS 331

Sterile container

Specimen Holding Temperature: Room temperature - 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 4 - 8 weeks
Notes: N/A
 Sputum (including endotracheal tube)  Culture and sensitivity (C&S)  MS 349

Sterile container or endotracheal tube

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
 Sputum (including endotracheal tube)  Fungus culture  MS 331

Sterile container or endotracheal tube

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes: N/A
 Sputum (including endotracheal tube)  Legionella culture   MS 331

Sterile container or endotracheal tube

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 2 - 3 weeks
Notes: N/A
 Sputum (including endotracheal tube)  Mycobacteria (AFB)  MS 331

Sterile container or endotracheal tube

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 4 - 8 weeks
Notes: N/A
Superficial wound swab and drainage  Culture and sensitivity (C&S)  MS 349

Copan Eswab and drainage in sterile container

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
 Throat swab  Corynebacterium diphtheriae  MS 331

Copan Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 1-2 weeks
Notes:N/A
 Throat swab  Culture and sensitivity (C&S)  MS 349

Cobas Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 24 - 48 hours
Notes:N/A
 Throat swab  Mycoplasma  MS 331

Cobas Eswab

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 2 weeks
Notes: N/A
Tissue and biopsy  Culture and sensitivity (C&S)  MS 349

Sterile container with sterile saline

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
Tissue and biopsy  Fungus culture  MS 331

Sterile container with sterile saline

Specimen Holding Temperature:

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours, Final: 4 weeks
Notes: N/A
Tissue and biopsy Mycobacteria (AFB)  MS 331

Sterile container with sterile saline

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 4 - 8 weeks
Notes: N/A
Tissue and biopsy  Viral detection   MS 331

Sterile container with sterile saline 

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 24 hours - 1 week
Notes: N/A
 Tympanocentesis fluid  Culture and sensitivity (C&S)  MS 349

Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes:N/A
 Tympanocentesis fluid  Fungus culture  MS 331

Sterile container 

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Fungal stain: 24 hours; Final: 4 weeks
Notes: N/A
 Tympanocentesis fluid  Mycobacteria (AFB)  MS 331

Sterile container

Specimen Holding Temperature: 4°C

Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: 4-8 weeks
Notes: N/A
Upper genital tract swab or aspirate Anaerobic culture and sensitivity (C&S) MS 349

 Anaerobic container

Specimen Holding Temperature: Room Temperature

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
Upper genital tract swab or aspirate Culture and sensitivity (C&S) MS 349

Copan Eswab

Specimen Holding Temperature: Room Temperature

Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Gram stain: same day;  Prelim: 24 hours;  Final: 48 - 72 hours
Notes: N/A
 Upper genital tract swab or aspirate  Mycobacteria (AFB) MS 331 

Sterile container

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: Provincial Health Lab
Expected Time for Results: Prelim: 1 week;  Final: 8 weeks
Notes: N/A
 Vaginal or vulvar swab  Group B streptococci (GBS) MS 349

Copan Eswab

Specimen Holding Temperature: 4°C

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: 48 hours
Notes: N/A

 Vaginal or vulvar swab  Trichomonas, Candida or bacterial vaginosis MS 349

Copan Eswab

Specimen Holding Temperature: Room Temperature

 Area: Bacteriology
Refer Out: N/A
Expected Time for Results: Same day
Notes: In-patient specimens are also examined for Group A strep and S. aureus.
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